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Code
OSN00004G
$240USD
ID Tag
Gt96-250808-G
Immunogen
Synthetic peptides from the c-terminal region of human nNOS conjugated to blue carrier protein was used as the antigen.
Accession
Also known
BNOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Constitutive NOS, NC-NOS, Nitric oxide synthase
Target
FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
CATALYTIC ACTIVITY: L-arginine + n NADPH + m O2 = citrulline + nitric oxide + n NADP+.
ENZYME REGULATION: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.
SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
TISSUE SPECIFICITY: Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum.
CATALYTIC ACTIVITY: L-arginine + n NADPH + m O2 = citrulline + nitric oxide + n NADP+.
ENZYME REGULATION: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.
SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
TISSUE SPECIFICITY: Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Bredt D.S, et al. Nature 351:714-718(1991).
Limitation
For research use only
Related
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Code
OSN00004G
$240USD
Unit size
500 ug
Conjugate
Unconjugated antibody
Host
Goat
Purity
IgG
Clonality
Polyclonal
Isotype
Polyclonal, IgG
Applications
IHC, WB. A concentration of 10-50 ug/ml is recommended. The optimal concentration should be determined by the end user.
Specificity
Specific for nNOS
Spcs X-react.
Rat, mouse, human, rabbit, guinea pig. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSN00004G
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSN00004G
$240USD
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IHC on paraffin sections of mouse brain tissue using Goat antibody to nNOS:OSN00004G . HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain tissue using Goat antibody to nNOS:OSN00004G . HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of mouse olfactory bulb tissue using Goat antibody to nNOS:OSN00004G . HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 100, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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