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Code
OST00110W
$240USD
ID Tag
Rb523-290608-WS
Immunogen
A synthetic peptide from the 4th cytoplasmic loop of human TRPM7 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Accession
Also known
Transient receptor potential cation channel subfamily M member 7, long transient receptor potential channel 7, LTrpC7, channel-kinase 1, CHAK1, LTRPC7, CHAK
Target
TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.
FUNCTION: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
CATALYTIC ACTIVITY: ATP + a protein: ADP + a phosphoprotein.
COFACTOR: Binds 1 zinc ion per subunit.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
FUNCTION: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
CATALYTIC ACTIVITY: ATP + a protein: ADP + a phosphoprotein.
COFACTOR: Binds 1 zinc ion per subunit.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ryazanova L.V, et al. J. Biol. Chem. 279:3708-3716(2004).
2. Ota T, et al. Nat. Genet. 36:40-45(2004).
2. Ota T, et al. Nat. Genet. 36:40-45(2004).
Limitation
For research use only
Code
OST00110W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 1000 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for TRPM7.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Code
OST00110W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00110W
$240USD
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WB on human neutrophil lysate using Rabbit antibody to TRPM7 (OST00110W) at a dilution of 1 : 200. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band. |
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IHC on paraffin sections of mouse olfactory bulb tissue using Rabbit antibody to TRPM7: OST00110W. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat cerebellum tissue using Rabbit antibody to TRPM7: OST00110W. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of mouse brain tissue using Rabbit antibody to TRPM7: OST00110W. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat cerebellum tissue using Rabbit antibody to TRPM7: OST00110W. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat cerebellum tissue using Rabbit antibody to TRPM7: OST00110W. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat cerebellum tissue using Rabbit antibody to TRPM7: OST00110W. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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