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Code
OSG00014W
$240USD
ID Tag
Rb476-290608-WS
Immunogen
A synthetic peptide from human basic FGF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species including mouse and chicken.
Accession
Also known
BFGF, FGFB, HBGF-2, prostatropin
Target
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Alexander S, et al. Arthritis Rheum. 56 (11), 3715-3725(2007).
Limitation
For research use only
Code
OSG00014W
$240USD
Unit size
150 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for FGF2.
Spcs X-react.
Human, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSG00014W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSG00014W
$240USD
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IHC on paraffin sections of human rectum using Rabbit antibody to FGF2 (150-200): OSG00014W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo's PT Module Detection was done using Novolink HRP polymer from Leica following manufacturer's instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human rectum using Rabbit antibody to FGF2 (150-200): OSG00014W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermos PT Module Detection was done using Novolink HRP polymer from Leica following manufacturers instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
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Rabbit antibody to FGF2
