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Code
OST00061W
$260USD
ID Tag
Rb432-150608-WS
Immunogen
A synthetic peptide from rat TRPA1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.
The location of the epitope is highlighted in green.
The location of the epitope is highlighted in green.
Accession
Also known
Transient receptor potential cation channel subfamily A member 1, ankyrin-like with transmembrane domains protein 1, transformation sensitive protein p120, ANKTM1
Target
The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.
TISSUE SPECIFICITY: Expressed in inner ear. In inner ear, it is found in the distal half of stereocilia, along the length of the kinocilium and at high level in the pericuticular zone (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion.
DEVELOPMENTAL STAGE: During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15.
Inhibited by ruthenium red, a potent blocker of TRPV channels.
TISSUE SPECIFICITY: Expressed in inner ear. In inner ear, it is found in the distal half of stereocilia, along the length of the kinocilium and at high level in the pericuticular zone (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion.
DEVELOPMENTAL STAGE: During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15.
Inhibited by ruthenium red, a potent blocker of TRPV channels.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Jordt S.-E, et al. Nature 427:260-265(2004).
Limitation
For research use only
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Code
OST00061W
$260USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 1000 is recommended for WB and 1:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. For WB, tissue lysates should be prepared in G3 lysing buffer (G3 lysing buffer contains only 1% Triton X-100 and no other detergents).
Specificity
Specific for TRPA1.
Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.
Format
Lyophilised. This product contains 0.02% benzalkonium chloride.
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
- Click to see the eLab Book
Code
OST00061W
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00061W
$260USD
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WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C. Click on eLAB BOOK tab to see the details. |
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