|
|
Code
OSV00001W
$260USD
ID Tag
Rb1170-260909-WS
Immunogen
A synthetic peptide from human VACHT conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.
Accession
Also known
Vesicular acetylcholine transporter, solute carrier family 18 member 3, SLC18A3, MGC12716
Target
FUNCTION: Involved in acetylcholine transport into synaptic vesicles.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus.
VACHT is also present in the olfactory bulb, nucleus of the lateral olfactory tract, olfactory tubercle.
SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus.
VACHT is also present in the olfactory bulb, nucleus of the lateral olfactory tract, olfactory tubercle.
SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ichikawa T, et al. J Chem Neuroanat. 13(1):23-39(1997).
Limitation
For research use only
Code
OSV00001W
$260USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 2000 is recommended for WB and to 1:1000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for VACHT.
Spcs X-react.
Human, mouse, rat, marmoset. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
- Click to see the eLab Book
Code
OSV00001W
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSV00001W
$260USD
|
|
IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
|
|
WB on rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
⚬ Products
Rabbit antibody to VACHT
