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Code
OSR00271W
$240USD
ID Tag
Rb1422-250410-WS
Immunogen
A synthetic peptide from human Runx1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Accession
Also known
Runx1, Core-binding factor, alpha 2 subunit, CBF-alpha 2, Acute myeloid leukemia 1 protein, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEBP2-alpha B, PEA2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, AML1, CBFA2
Target
FUNCTION: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
SUBUNIT: Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with MYST3 and MYST4. Interacts with SUV39H1, leading to abrogate the transactivating and DNA-binding properties of RUNX1.
TISSUE SPECIFICITY: Highest levels in thymus, bone marrow and peripheral blood.
SUBUNIT: Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with MYST3 and MYST4. Interacts with SUV39H1, leading to abrogate the transactivating and DNA-binding properties of RUNX1.
TISSUE SPECIFICITY: Highest levels in thymus, bone marrow and peripheral blood.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Miyoshi H, et al. Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
Limitation
For research use only
Related
products
products
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Code
OSR00271W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. For WB, the thymus lysate should be prepared in G2 lysing buffer (G2 contains 1% Triton X-100, 1% SDC).
Specificity
Specific for Runx1.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSR00271W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
⚬ Products
Rabbit to antibody to Runx1
