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Code
OSS00035W
$240USD
ID Tag
Rb324-200308-WS
Immunogen
A synthetic peptide from aa region 10-60 of human VAMP2 conjugated to blue carrier protein was used as the antigen. The VAMP2 peptide shares 93% shared identity with VAMP1. The peptide is homologous in many other species including rat, mouse and xenopus.
Accession
Also known
VAMP-2, Synaptobrevin-2, VAMP2, SYB2.
VAMP1, Synaptobrevin-1
Target
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.
FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Note: Neuronal synaptic vesicles.
FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Note: Neuronal synaptic vesicles.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Archer B.T, et al. J. Biol. Chem. 265:17267-17273(1990).
Limitation
For research use only
Related
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Code
OSS00035W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for VAMP2 and VAMP1.
Spcs X-react.
Human, rat, mouse, marmoset. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSS00035W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSS00035W
$240USD
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IF on 1 day- old mouse cortical cell culture using Rabbit antibody to VAMP 2, 1 (OSS00035W) at 1: 1000 dilution. DAPI counter stained. |
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WB on rat brain lysate using Rabbit antibody to VAMP 2,1 (10-60)(OSS00035W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide abolishes the detected band. |
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IHC on rat brain (cortex) using Rabbit antibody to VAMP 2,1 (10-60) (OSS00035W) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown). |
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