Code

GSC00002P

$120USD   

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ID Tag

U9483DD180-4

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Unit size

50 ug

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Accession

CAMP_HUMAN

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Also known

CAMP, CAP18, FALL39, FALL-39, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18, LL37, CRAMP, HSD26

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Target

This is the immunising peptide for the product Rabbit antibody to human Cathelicidin antimicrobial peptide  (OSC00009W).

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Purity

>94%

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Applications

Control peptide

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Format

Lyophilised

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Reconstitution

This peptide is soluble in PBS and water.

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Storage

Maintain refrigerated at 2-8C for up to 12 months. After reconstitution keep at -20C. Avoid freeze and thaw cycles.

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Expiry Date

12 months after reconstitution

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Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

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Limitation

For research use only

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Related Products

OSC00009W,OSC00028G

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- Click to see the eLab Book

IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IF on ethanol-fixed human spermatosoid using Rabbit antibody to LL-37 (OSC00009W) at 1: 100 dilution.

IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human lung tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human kidney tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human fallopian tube tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human appendix tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human thyroid tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)

IHC on paraffin sections of human thyroid tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human thyroid tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)

IHC on paraffin sections of human liver tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human liver tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human placenta tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human prostate tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human skin tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human thyroid tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Rabbit antibody to LL-37 (OSC00009W), diluted 1 : 200 in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclar cells.

WB on different lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

IHC on paraffin sections of human large intestine tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human tonsil tissue using Rabbit antibody to LL-37: OSC00009W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.