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Code
OSA00033W
$240USD
ID Tag
Rb270-270108-WS
Immunogen
A synthetic peptide from aa region 20-80 of human ATG4B conjugated to an immunogenic carrier protein has been used as the antigen. The antigen is homologous in mouse.
Accession
Also known
AUTL1, Autophagin-1, AUTL1, cysteine protease ATG4B, AUT-like 1 cysteine endopeptidase, Autophagy-related protein 4 homolog B
Target
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
ENZYME REGULATION: Inhibited by N-ethylmaleimide
SUBCELLULAR LOCATION: Cytoplasm
ENZYME REGULATION: Inhibited by N-ethylmaleimide
SUBCELLULAR LOCATION: Cytoplasm
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Marino G, et al. J. Biol. Chem. 278:3671-3678(2003).
2. Olsen J.V, et al. Cell 127 (3), 635-648(2006).
2. Olsen J.V, et al. Cell 127 (3), 635-648(2006).
Limitation
For research use only
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Code
OSA00033W
$240USD
Unit size
150 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for ATG4B.
Spcs X-react.
Human, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00033W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSA00033W
$240USD
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WB on human neutrophil lysate using Rabbit antibody to ATG4B (OSA00033W) at a dilution of 1 : 100. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band. |
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Rabbit antibody to ATG4B
