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Code
OSA00018W
$240USD
ID Tag
Rb303-010308-WS
Immunogen
A synthetic peptide from human autophagy-related protein 3 (ATG3, APG3, APG3L, APG3-like, hApg3, protein PC3-96) conjugated to an immunogenic carrier protein has been used as the antigen.
Accession
Also known
ATG3, APG3, APG3L, APG3-like, protein PC3-96
Target
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy.
FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy.
SUBCELLULAR LOCATION: Cytoplasm
TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy.
SUBCELLULAR LOCATION: Cytoplasm
TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Tanida I, et al. FEBS J. 273 (11), 2553-2562(2006).
2. Brill L.M, et al. Anal. Chem. 76 (10), 2763-2772(2004).
3. Tanida I, et al. J. Biol. Chem. 277 (16), 13739-13744(2002).
2. Brill L.M, et al. Anal. Chem. 76 (10), 2763-2772(2004).
3. Tanida I, et al. J. Biol. Chem. 277 (16), 13739-13744(2002).
Limitation
For research use only
Related
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Code
OSA00018W
$240USD
Unit size
150 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. Use at a dilution of 1 : 300 to 1 : 2000. The optimal dilution should be determined by the end user.
Specificity
Specific for ATG3.
Spcs X-react.
Human, mouse and rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00018W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
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Rabbit antibody to ATG3
