Rb302-010308-WS

A synthetic peptide from human cysteine protease ATG4C (autophagin-3, APG4C) conjugated to an immunogenic carrier protein has been used as the antigen.

ATG4C_HUMAN
ATG4C_RAT
ATG4C_MOUSE

ATG4C, autophagin-3, APG4C, AUTL1, AUTL3, APG4-C, ATG4 autophagy related 4 homolog C (S. cerevisiae), Autophagy-related protein 4 homolog C, Autophagy-related cysteine endopeptidase 3, AUT-like 3 cysteine endopeptidase

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
ENZYME REGULATION: Inhibited by N-ethylmaleimide.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, liver and testis.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Marino G, et al. J. Biol. Chem. 282 (25), 18573-18583(2007).
2. Marino G, et al. J. Biol. Chem. 278 (6), 3671-3678(2003).

For research use only

	Code Product Name OSA00004W Sheep antibody to ATG4C OSA00013G Sheep antibody to ATG4C

150 µl

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user.

Specific for ATG4C.

Human, mouse and rat. Other species not yet tested.

Lyophilised

Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

- Click to download the Data Sheet

- Click to download the Material Safety Data Sheet