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Code
OST00033W
$240USD
ID Tag
Sh238-1-280310-WS
Immunogen
A synthetic peptide from human TRPM4 conjugated to blue carrier protein was used as the antigen.
Accession
Also known
Transient receptor potential cation channel subfamily M member 4, long transient receptor potential channel 4, hTRPM4, melastatin-4, calcium-activated non-selective cation channel 1, TRPM4, TRPM4B, FLJ20041
Target
FUNCTION: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.
ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2).
SUBUNIT: Homomultimer.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed with a high expression in intestine and prostaste. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells.
ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2).
SUBUNIT: Homomultimer.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed with a high expression in intestine and prostaste. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Hofmann T, et al. Curr. Biol. 13:1153-1158(2003).
Limitation
For research use only
Code
OST00033W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
Sheep
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for TRPM4.
Spcs X-react.
Human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OST00033W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00033W
$240USD
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A cell cytospin of human lung cancer cell line A549 was fixed with cold acetone for 90 seconds and air-dried. Cells were incubated with the blocking buffer (PBS containing 5% FCS) for 30 minutes at room temperature. Cells were then washed once in PBS and incubated with primary antibody, diluted 1:100 in the blocking buffer, for 30 minutes. Slides were washed 3X in PBS and incubated with Goat anti-sheep conjugated to Alexa-488, diluted 1:200 in blocking buffer, for 30 minutes at room temperature in the dark. Slides were washed as above and mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml was used for nuclear counterstaining. Fluorescent cell staining were analysed using a Olympus microscope and the analysis LS Research software. |
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Sheep antibody to TRPM4
