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Code
OSG00009W
$240USD
ID Tag
Rb1451-120610-WS
Immunogen
A synthetic peptide from n-terminal region of human GABARAP conjugated to an immunogenic carrier protein has been used as the antigen.
Accession
Also known
GABA(A) receptor-associated protein, GABARAP
Target
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed.
GABARAP belongs ot a large family of proteins that mediate intracellular membrane trafficking and/or fusion. GABARAP binds not only to GABA, type A but also to tubulin, gephrin, and ULK1.
May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. It interacts with GPHN and NSF; interacts with GABRG2, beta-tubulin and ULK1.
SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoplasm, cytoskeleton.
Note: Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
TISSUE SPECIFICITY: Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
GABARAP belongs ot a large family of proteins that mediate intracellular membrane trafficking and/or fusion. GABARAP binds not only to GABA, type A but also to tubulin, gephrin, and ULK1.
May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. It interacts with GPHN and NSF; interacts with GABRG2, beta-tubulin and ULK1.
SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoplasm, cytoskeleton.
Note: Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
TISSUE SPECIFICITY: Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Kawaguchi S.Y, et al. J. Neurosci. 27 (25), 6788-6799(2007).
2. Lou X.Y, et al. Hum. Mol. Genet. 16 (2), 142-153(2007).
2. Lou X.Y, et al. Hum. Mol. Genet. 16 (2), 142-153(2007).
Limitation
For research use only
Code
OSG00009W
$240USD
Unit size
150 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. Use at a dilution of 1 : 100 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for GABARAP.
Spcs X-react.
Human, mouse and rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSG00009W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSG00009W
$240USD
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WB on 293 cell lysate using Rabbit antibody to GABARAP (OSG00009W) at 1 : 200 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to GABARAP (OSG00009W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/No and counterstained with hematoxylin. |
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IHC on rat spinal cord (paraffin section) using Rabbit antibody to GABARAP (OSG00009W) at 1 : 200 dilution incubated overnight at 4C, developed with DAB/No and counterstained with hematoxylin. |
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