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Code
DOM00001G
$240USD
ID Tag
Rb2141-040913-G
Immunogen
Native myeloperoxidase isolated from human leucocytes
Accession
Also known
Myeloperoxidase
Target
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils.
SUBCELLULAR LOCATION: Lysosome
SUBCELLULAR LOCATION: Lysosome
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Morishita K, et al. J. Biol. Chem. 262:3844-3851(1987).
Limitation
For research use only
Related
products
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Code
DOM00001G
$240USD
Unit size
500 ug
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Protein G purified IgG
Clonality
Polyclonal
Isotype
Polyclonal, IgG
Applications
IHC, WB. A working concentration of 10-50 ug/ml is recommended. The optimal dilution should be determined by the end user. This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins.
Specificity
Specific for MPO.
Spcs X-react.
Human
Format
Lyophilised
Reconstitution
Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
DOM00001G
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
DOM00001G
$240USD
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Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analysed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X |
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Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analysed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X |
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10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences). |
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Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X |
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