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IHC-P on paraffin sections of mouse spinal cord using Rabbit antibody to TMC3 (OSR00226W).
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin. |
IHC-P on paraffin sections of mouse spinal cord using Rabbit antibody to TMC3 (OSR00226W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
WB on mouse tongue lysate using Rabbit antibody to TMC3 (OSR00226W). Dilution: 1: 500, incubated overnight at 4C in MPX from LiCore. Blocking buffer: 0.2 µm filtered 0.5% LFDM in 1x PBS containing 0.01% Tween-20 Washing buffer: 1x PBS Detection: ECL using C-Digit from LiCore |