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Code
OSR00146W
$260USD
ID Tag
Rb156-251107-WS
Immunogen
A synthetic peptide from the 3d cytoplasmic domain of human GPR143 (OA1) conjugated to blue carrier protein was used as the antigen.
Accession
Also known
G-protein coupled receptor 143, ocular albinism type 1 protein, GPR143, OA1
Target
Ocular albinism type 1 protein is a conserved integral membrane protein with seven transmembrane domains. It is expressed in the eye and epidermal melanocytes.
FUNCTION: Not known; binds heterotrimeric G proteins.
SUBCELLULAR LOCATION: Melanosome membrane; Multi-pass membrane protein. Note: Targeted to intracellular organelles, namely the melanosomes in pigment cells.
TISSUE SPECIFICITY: Exclusively expressed in pigment cells.
DISEASE: Defects in GPR143 are the cause of ocular albinism type 1 (OA1); also known as Nettleship-Falls type ocular albinism. OA1 is an X-linked disorder characterized by severe impairment of visual acuity, retinal hypopigmentation and the presence of macromelanosomes.
FUNCTION: Not known; binds heterotrimeric G proteins.
SUBCELLULAR LOCATION: Melanosome membrane; Multi-pass membrane protein. Note: Targeted to intracellular organelles, namely the melanosomes in pigment cells.
TISSUE SPECIFICITY: Exclusively expressed in pigment cells.
DISEASE: Defects in GPR143 are the cause of ocular albinism type 1 (OA1); also known as Nettleship-Falls type ocular albinism. OA1 is an X-linked disorder characterized by severe impairment of visual acuity, retinal hypopigmentation and the presence of macromelanosomes.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Bassi M.T, et al. Nat. Genet. 10:13-19(1995).
Limitation
For research use only
Code
OSR00146W
$260USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for OA1.
Spcs X-react.
Human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSR00146W
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSR00146W
$260USD
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IHC-P on paraffin sections of human eye. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of human eye. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of human eye. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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