Rb1485-120610-WS

A synthetic peptide from aa region 110-160 of human ASIC4 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

ASIC4_HUMAN
ASIC4_RAT
ASIC4_MOUSE

Amiloride sensitive cation channel 4 pituitary, acid-sensing ion channel 4, spinal chord ASIC, Spasic, BNAC4, MGC17248, MGC24860

FUNCTION: Probable cation channel with high affinity for sodium. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception.
SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: This gene is predominantly expressed in the pituitary gland. Expressed in brain, spinal chord and dorsal root ganglion (DRG). Expressed by a subset of sensory neurons in the DRG. Expressed by granule cells in the cerebellar cortex. In hippocampus, expression is detected in dentate gyrus granule cells, in pyramidal cells of CA1-CA3 subfields and in interneurons of the striatum oriens and radiatum of all subfields. In cerebral cortex expressed in small, medium and large pyramidal cells in layers 2, 3 and 5 respectively. Also expressed in striatum, globus pallidus, inferior and superior calliculi, amygdala, magnocellular preoptic nucleus, islands of Calleja and large neurons of olfactory tubercules.
DEVELOPMENTAL STAGE: Highly expressed in newborn spinal chord but hardly detected in the cerebellum compared to adult. Expressed at postnatal day 1 in ependymal cells lining the central canal of spinal chord and in motor neurons. In adult, expression decreases in ependymal cells and increases in motor neurons. The number of positive interneurons decreases but the individual interneuron expression increases in adult spinal chord compared to newborn.
MISCELLANEOUS: In vitro, has no proton-gated channel activity.

Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Gruender S, et al. NeuroReport 11:1607-1611(2000).

For research use only

100 ul

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specific for ASIC4.

Human, rat and mouse. Other species not yet tested.

Lyophilised

Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

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