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Code
OSE00046G
$260USD
ID Tag
Gp3169-070524-WS
Immunogen
Recombinant truncated EGFP/GFP/Venus protein was used as the antigen.
Accession
Also known
Enhanced Green Fluorescence Protein
Target
GFP is a naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al.
GFP makes green light out of aequorinâs blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen.
BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
GFP makes green light out of aequorinâs blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen.
BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Tsien R. Y. Annu. Rev. Biochem. 67:509-544(1998).
Limitation
For research use only
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Code
OSE00046G
$260USD
Unit size
500 ug
Conjugate
Unconjugated antibody
Host
Guinea pig
Purity
IgG, ammonium sulfate cut
Clonality
Polyclonal
Isotype
Polyclonal, mainly IgG
Applications
IHC, WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user.
Specificity
Specific for EGFP/GFP/Venus
Spcs X-react.
Any organism expressing EGFP/GFP/Venus.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of DI sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSE00046G
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
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Guinea pig antibody to EGFP/GFP/Venus
