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Code
OSA00295W
$260USD    Buy Now 

ID Tag
Gp2988-06112023-WS

Immunogen
A synthetic peptide from rat AATC conjugated to blue carrier protein was used as the antigen.

Accession

Also known
AATC, Cysteine aminotransferase, Cysteine transaminase, Glutamate oxaloacetate transaminase 1, Transaminase A

Target
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Edward L Huttlin et al. Cell. 2010 Dec 23;143(7):1174-89

Limitation
For research use only

Related
products

Code
OSA00295W
$260USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
Guinea pig

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 500 is recommended for IHC-P and WB. The optimal dilution should be determined by the end user.

Specificity
Specific for AATC

Spcs X-react.
Rat, mouse, human

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSA00295W
$260USD    Buy Now 


Guinea pig antibody to Aspartate Aminotransferase (AATC) WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:500 incubated at 4C overnight then Osenses' Rb anti Gp adaptobody at 1:3000 dilution, rinsed followed by Sigma's HRP Goat anti Rb. Click on eLAB BOOK tab to see the details.

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Guinea pig antibody to Aspartate Aminotransferase (AATC)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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