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Code
OST00484W
$240USD
ID Tag
Gp0526-17112020-WS
Immunogen
A synthetic peptide from aa region 2-50 of human TMPRSS2 conjugated to blue carrier protein was used as the antigen.
Accession
Also known
Transmembrane protease serine 2, Epitheliasin, Plasmic transmembrane protein X
Target
Function: Plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate.
Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells.
In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia.
(Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry.
Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.
Expression: Expressed in several tissues that comprise large populations of epithelial cells with the highest level of transcripts measured in the prostate gland. Expressed in type II pneumocytes in the lung (at protein level). Expressed strongly in small intestine. Also expressed in colon, stomach and salivary gland. Coexpressed with ACE2 within lung type II pneumocytes, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Gastroenterology 136:872-882(2009).
Limitation
For research use only
Related
products
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Code
OST00484W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
Guinea pig
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 2000 is recommended fro WB and IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for TMPRSS2.
Spcs X-react.
Human, rat. Other species not yet test.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OST00484W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00484W
$240USD
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IHC-P on paraffin sections of rat kidney.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
Detection was done using Osenses' Rabbit anti Gp adaptobody at 1:3000 dilution, then Sigma's HRP-goat anti Rb; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 1000, incubated 30 min at RT.
Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat kidney. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Osenses' Rabbit anti Gp adaptobody at 1:3000 dilution, then Sigma's HRP-goat anti Rb; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat kidney. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Osenses' Rabbit anti Gp adaptobody at 1:3000 dilution, then Sigma's HRP-goat anti Rb; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat liver (no staining observed). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Osenses' Rabbit anti Gp adaptobody at 1:3000 dilution, then Sigma's HRP-goat anti Rb; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat liver (no staining observed). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Osenses' Rabbit anti Gp adaptobody at 1:3000 dilution, then Sigma's HRP-goat anti Rb; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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WB on kidney lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight then Osenses' Rb anti Gp adaptobody at 1:3000 dilution, rinsed followed by Sigma's HRP Goat anti Rb. Click on eLAB BOOK tab to see the details. |
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Guinea pig antibody to TMPRSS2
