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Code
OSV00111W
$260USD
ID Tag
Gp0542-061220-WS
Immunogen
A synthetic peptide from aa region 470-517 of mouse VMAT2 conjugated to blue carrier protein was used as the antigen.
Accession
Also known
VMAT2, Synaptic vesicular amine transporter, VAT2, Monoamine transporter, Solute carrier family 18 member 2, SLC18A2, SVMT, VMAT2
Target
FUNCTION: Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein.
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Carninci P, et al. Science 309:1559-1563(2005)
Limitation
For research use only
Related
products
products
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Code
OSV00111W
$260USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
Guinea pig
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1:200 to 1:1000 is recommended for IHC-P and 1:000-1:2000 for WB. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for VMAT2.
Spcs X-react.
Mouse, rat. Other species not yet tested but likely to work in human.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OSV00111W
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSV00111W
$260USD
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WB on tissue lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:500 dilution incubated overnight at 4C. Click on eLAB Book tab to see the details. |
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IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse hippocampus. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Sigma's HRP-rabbit anti Gp; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin. |
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Guinea pig antibody to VMAT2
