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Code
OSC00321W
$240USD
ID Tag
Rb2458-081014-WS
Immunogen
A synthetic peptide from human Clavesin-1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Accession
Also known
Clavesin-1, Clavesin1, Clavesin 1, CLVS1, Cellular retinaldehyde-binding protein-like, Retinaldehyde-binding protein 1-like 1
Target
FUNCTION: Required for normal morphology of late endosomes and/or lysosomes in neurons (By similarity). Binds phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
SUBCELLULAR LOCATION: Golgi apparatus ⺠trans-Golgi network membrane; Peripheral membrane protein. Cytoplasmic vesicle ⺠clathrin-coated vesicle. Early endosome membrane; Peripheral membrane protein.
Tissue specificity: Expressed mainly in the brain.
SUBCELLULAR LOCATION: Golgi apparatus ⺠trans-Golgi network membrane; Peripheral membrane protein. Cytoplasmic vesicle ⺠clathrin-coated vesicle. Early endosome membrane; Peripheral membrane protein.
Tissue specificity: Expressed mainly in the brain.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Kong Y.-H. et al. Biotechnol. Lett. 28:1327-1333(2006)
Limitation
For research use only
Code
OSC00321W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.
Specificity
Specific for Clavesin 1.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSC00321W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSC00321W
$240USD
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IHC on paraffin sections of rat DRG tissue using Rabbit antibody to Clavesin 1 (300-354): OSC00321W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Clavesin 1 (300-354): OSC00321W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\\\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Clavesin 1 (300-354): OSC00321W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\\\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat DRG tissue using Rabbit antibody to Clavesin 1 (300-354): OSC00321W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\\\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat spinal cord tissue using Rabbit antibody to Clavesin 1 (300-354): OSC00321W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\\\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat spinal cord tissue using Rabbit antibody to Clavesin 1 (300-354): OSC00321W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\\\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat olfactory bulb tissue using Rabbit antibody to Clavesin 1 (300-354): OSC00321W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\\\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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WB on rat brain lysate using Rabbit antibody to Clavesin 1 (300-354): OSC00321W at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20 |
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Rabbit antibody to Clavesin 1 (300-354)
