NMDAR3A antibody, NR3A antibody

Primary Antibodies

Peptides

Histology / Detection

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OVERVIEW
SPECS
CONTROL PEPTIDE
MSDS/DS
IMAGES

Code

OSN00101W

$240USD

ID Tag

Rb2343-290414-WS

Immunogen

A synthetic peptide from aa region 990-1040 of rat NMDAR3A conjugated to blue carrier protein was used as the antigen. The peptide shares 95% identity with mouse sequence.

Accession

NMDAR3A_RAT
NMDAR3A_MOUSE

Also known

NMD3A, Glutamate [NMDA] receptor subunit 3A, Glutamate receptor chi-1, N-methyl-D-aspartate receptor, N-methyl-D-aspartate receptor subtype 3A, NR3A, NMDAR-L, NMDAR-L1

Target

FUNCTION: NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. May play a role in the development of dendritic spines. May also play a role in PPP2CB-NMDAR mediated signaling mechanism.
Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction âº synapse âº postsynaptic cell membrane. Cell junction âº synapse âº postsynaptic cell membrane âº postsynaptic density. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane.
Tissue specificity: Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Ciabarra A.M, et al. J. Neurosci. 15:6498-6508(1995)
2. Sucher N.J, et al. J. Neurosci. 15:6509-6520(1995)

Limitation

For research use only

Code

OSN00101W

$240USD

Unit size

100 µl

Conjugate

Unconjugated antibody

Host

NZ white rabbit

Purity

Whole serum

Clonality

Polyclonal

Isotype

Polyclonal, whole serum

Applications

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity

Specific for NMDAR3A.

Spcs X-react.

Rat. Other species not yet tested but expected to work in mouse.

Format

Lyophilised

Reconstitution

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code

OSN00101W

$240USD

PDF Data Sheet

- Click to download the Data Sheet

MSDS

Click to download the data sheet - Click to download the Material Safety Data Sheet

Code

OSN00101W

$240USD

IHC on paraffin sections of rat spinal cord tissue using Rabbit antibody to NMDAR3A (990-1040): OSN00101W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of rat spinal cord tissue using Rabbit antibody to NMDAR3A (990-1040): OSN00101W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

⚬ Products

Rabbit antibody to NMDAR3A (990-1040)