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Code
OSN00100W
$240USD
ID Tag
Rb2342-290414-WS
Immunogen
A synthetic peptide from aa region 1091-1135 of rat NMDAR3A conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and shares 92% identity with human sequence.
Accession
Also known
NMD3A, Glutamate [NMDA] receptor subunit 3A, Glutamate receptor chi-1, N-methyl-D-aspartate receptor, N-methyl-D-aspartate receptor subtype 3A, NR3A, NMDAR-L, NMDAR-L1
Target
FUNCTION: NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. May play a role in the development of dendritic spines. May also play a role in PPP2CB-NMDAR mediated signaling mechanism.
Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane. Cell junction synapse postsynaptic cell membrane postsynaptic density. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane.
Tissue specificity: Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord.
Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane. Cell junction synapse postsynaptic cell membrane postsynaptic density. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane.
Tissue specificity: Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ciabarra A.M, et al. J. Neurosci. 15:6498-6508(1995)
Limitation
For research use only
Code
OSN00100W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for NMDAR3A.
Spcs X-react.
Rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSN00100W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSN00100W
$240USD
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IHC on paraffin sections of rat olfactory bulb tissue using Rabbit antibody to NMDAR3A (1091-1135): OSN00100W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat olfactory bulb tissue using Rabbit antibody to NMDAR3A (1091-1135): OSN00100W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat olfactory bulb tissue using Rabbit antibody to NMDAR3A (1091-1135): OSN00100W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat brain tissue using Rabbit antibody to NMDAR3A (1091-1135): OSN00100W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat olfactory bulb tissue using Rabbit antibody to NMDAR3A (1091-1135): OSN00100W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat olfactory bulb tissue using Rabbit antibody to NMDAR3A (1091-1135): OSN00100W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat olfactory bulb tissue using Rabbit antibody to NMDAR3A (1091-1135): OSN00100W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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Rabbit antibody to NMDAR3A (1091-1135)
