Code
OSR00377W
$240USD
ID Tag
Rb2143-011013-WS
Immunogen
A synthetic peptide from aa region 750-800 of mouse Rubicon conjugated to an immunogenic carrier protein has been used as the antigen. The peptide is homologous in human and rat.
Accession
Also known
Run domain Beclin 1 interacting and cysteine rich containing protein, Baron
Target
Function: Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity.
Subcellular location: Late endosome. Lysosome. Early endosome. Note: Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Matsunaga K, et al. Nat. Cell Biol. 11:385-396(2009)
Limitation
For research use only
Code
OSR00377W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. Use at a dilution of 1 : 200 to 1 : 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for Rubicon.
Spcs X-react.
Mouse, human, rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.