Rb2089-090613-WS

Synthetic peptide "KGGPSTEGVPKSMREEYRKEQQSAVD" from aa region 60-85 of Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) Matrix protein 2 conjugated to blue carrier protein was used as the antigen.

Proton channel protein M2, M

Function: Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation.
Cytoplasmic tail plays an important role in virion assembly and morphogenesis
Subcellular location: Virion membrane. Host apical cell membrane; Single-pass type III membrane protein. Note: Abundantly expressed at the apical plasma membrane in infected polarized epithelial cells, in close proximity to budding and assembled virions. Minor component of virions (only 16-20 molecules/virion).

Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Zharikova D, et al. J. Virol. 79:6644-6654(2005)

For research use only

100 ul

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specific for Matrix protein 2.

Influenza A virus

Lyophilised

Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

- Click to download the Data Sheet

- Click to download the Material Safety Data Sheet