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Code
OSA00214W
$240USD
ID Tag
Rb1959-091212-WS
Immunogen
A synthetic peptide from human ABCG1 conjugated to blue carrier protein was used as the antigen.
Accession
Also known
ATP-binding cassette sub-family G member 2, Breast cancer resistance protein, CDw338, Mitoxantrone resistance-associated protein, Placenta-specific ATP-binding cassette transporter, CD338, ABCP, BCRP, BCRP1, MXR
Target
FUNCTION: Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Subcellular location: Cell membrane; Multi-pass membrane protein
Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.
Subcellular location: Cell membrane; Multi-pass membrane protein
Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Zhou S, et al. Nat. Med. 7:1028-1034(2001)
Limitation
For research use only
Code
OSA00214W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for ABCG2.
Spcs X-react.
Human, marmoset, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OSA00214W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSA00214W
$240USD
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WB on tissues and cell line lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
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WB on tissue lysates prepared in different lysing buffers. Note the profound effect of the composition of the lysing buffers on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody dilutions: 1:1000 in Spleen, Skin, Brain; 1:2000 in Testis incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
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Rabbit antibody to ABCG2
