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Code
OSV00096W
$240USD
ID Tag
Rb1817-240612-WS
Immunogen
A synthetic peptide from aa region 1-70 of mouse VAMP8 conjugated to an immunogenic carrier protein was used as the antigen.
Accession
Also known
VAMP8, VAMP-8, Endobrevin
Target
Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
Subcellular location: Membrane; Single-pass type IV membrane protein
Tissue specificity: Platelets. Expressed abundantly in the kidney, less in the liver, brain, kidney, heart, lung, pancreas and placenta.
Subcellular location: Membrane; Single-pass type IV membrane protein
Tissue specificity: Platelets. Expressed abundantly in the kidney, less in the liver, brain, kidney, heart, lung, pancreas and placenta.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Wong S.H, et al. Mol. Biol. Cell 9:1549-1563(1998)
2. Hillier L.W, et al. Nature 434:724-731(2005)
3. Polgar J, et al. Blood 100:1081-1083(2002)
2. Hillier L.W, et al. Nature 434:724-731(2005)
3. Polgar J, et al. Blood 100:1081-1083(2002)
Limitation
For research use only
Related
products
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Code
OSV00096W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for VAMP8.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSV00096W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSV00096W
$240USD
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IHC-P on paraffin sections of rat kidney. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat lung. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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