Anti ATP8B1 antibody, Probable phospholipid-transporting ATPase IC antibody

Primary Antibodies

ABC Transporters

Autophagy

Cancer

Cellular

Immunology

Reporter Genes & Tags

Reproduction

SLC & Regulators

Subcellular Markers

Toxins

Histology / Detection

⚬ Services

OVERVIEW
SPECS
CONTROL PEPTIDE
MSDS/DS
IMAGES

Code

OSA00164W

$265USD

ID Tag

Rb1729-020111-WS

Immunogen

A synthetic peptide from c-terminal region of human ATP8B1 conjugated to blue carrier protein was used as the antigen.

Accession

ATP8B1_HUMAN
ATP8B1_MOUSE
ATP8B1_RAT

Also known

Probable phospholipid-transporting ATPase IC, ATPase class I type 8B member 1, Familial intrahepatic cholestasis type 1, ATPIC, FIC1, PFIC

Target

FUNCTION: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May play a role in asymmetric distribution of phospholipids in the canicular membrane. May have a role in transport of bile acids into the canaliculus, uptake of bile acids from intestinal contents into intestinal mucosa or both. In cooperation with ABCB4 may be involved in establishing integrity of the canalicular membrane thus protecting hepatocytes from bile salts. Together with TMEM30A is involved in uptake of the synthetic drug alkylphospholipid perifosine. Involved in the microvillus formation in polarized epithelial cells; the function seems to be independent from its flippase activity. Required for the preservation of cochlear hair cells in the inner ear. May act as cardiolipin transporter during inflammatory injury.
Subcellular location: Membrane; Multi-pass membrane protein
Tissue specificity: Found in most tissues except brain and skeletal muscle. Most abundant in pancreas and small intestine.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Bull L.N, et al. Nat. Genet. 18:219-223(1998)

Limitation

For research use only

Code

OSA00164W

$265USD

IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1: OSA00164W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human muscle tissue (negative control) using Rabbit antibody to ATP8B1: OSA00164W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human bowel tissue using Rabbit antibody to ATP8B1: OSA00164W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.

⚬ Products

Rabbit antibody to ATP8B1