NMDAR3A antibody, NR3A antibody

Primary Antibodies

Peptides

Histology / Detection

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OVERVIEW
SPECS
CONTROL PEPTIDE
MSDS/DS
IMAGES

Code

OSN00066W

$240USD

ID Tag

Rb1702-021210-WS

Immunogen

A synthetic peptide from aa region 800-850 of extracellular domain of rat NMDAR3A conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and shares 95% identity with human sequence.

Accession

NMDAR3A_RAT
NMDAR3A_MOUSE

Also known

NMD3A, Glutamate [NMDA] receptor subunit 3A, Glutamate receptor chi-1, N-methyl-D-aspartate receptor, N-methyl-D-aspartate receptor subtype 3A, NR3A, NMDAR-L, NMDAR-L1

Target

FUNCTION: NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. May play a role in the development of dendritic spines. May also play a role in PPP2CB-NMDAR mediated signaling mechanism.
Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction âº synapse âº postsynaptic cell membrane. Cell junction âº synapse âº postsynaptic cell membrane âº postsynaptic density. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane.
Tissue specificity: Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Ciabarra A.M, et al. J. Neurosci. 15:6498-6508(1995)
2. Sucher N.J, et al. J. Neurosci. 15:6509-6520(1995)

Limitation

For research use only

Code

OSN00066W

$240USD

Unit size

100 µl

Conjugate

Unconjugated antibody

Host

NZ white rabbit

Purity

Whole serum

Clonality

Polyclonal

Isotype

Polyclonal, whole serum

Applications

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity

Specific for NMDAR3A.

Spcs X-react.

Rat, mouse. Other species not yet tested.

Format

Lyophilised

Reconstitution

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code

OSN00066W

$240USD

PDF Data Sheet

- Click to download the Data Sheet

MSDS

Click to download the data sheet - Click to download the Material Safety Data Sheet

Code

OSN00066W

$240USD

IHC on paraffin sections of human lung tissue using Rabbit antibody to NMDAR3A (800-850): OSN00066W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human appendix tissue using Rabbit antibody to NMDAR3A (800-850): OSN00066W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human liver tissue using Rabbit antibody to NMDAR3A (800-850): OSN00066W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of human tonsil tissue using Rabbit antibody to NMDAR3A (800-850): OSN00066W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

⚬ Products

Rabbit antibody to NMDAR3A (800-850)