SAP97 antibody, SAP 97 antibody, DLG1 antibody

OVERVIEW
SPECS
CONTROL PEPTIDE
MSDS/DS
IMAGES

Code

OSD00038W

$240USD

ID Tag

Rb1572-120910-WS

Immunogen

A synthetic peptide from aa region 2-50 of human SAP97 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

SAP97_HUMAN
SAP97_RAT
SAP97_MOUSE

Also known

DLG1, Disks large homolog 1, Synapse-associated protein 97

Target

Function: Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
Subcellular location: Membrane; Peripheral membrane protein. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction âº synapse âº postsynaptic cell membrane âº postsynaptic density. Cell junction âº synapse. Cell membrane âº sarcolemma. Note: Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities.
Tissue specificity: Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Kim E, et al. Nature 378:85-88(1995)
2. Lue R.A, et al. J. Cell Biol. 135:1125-1137(1996)

Limitation

For research use only

Code

OSD00038W

$240USD

IHC on paraffin sections of rat heart tissue using Rabbit antibody to SAP97 (2-50): OSD00038W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of rat kidney tissue using Rabbit antibody to SAP97 (2-50): OSD00038W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of rat brain tissue using Rabbit antibody to SAP97 (2-50): OSD00038W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of rat lung tissue using Rabbit antibody to SAP97 (2-50): OSD00038W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

⚬ Products

Rabbit antibody to SAP97 (2-50)