|
|
Code
OSO00050W
$240USD
ID Tag
Rb1455-120610-WS
Immunogen
A synthetic peptide from c-terminal region of mouse Orexin receptor 2 (Ox2r) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in human and rat.
Accession
Also known
Orexin receptor type 2, Ox2r, Hypocretin receptor type 2, HCRTR2
Target
FUNCTION: Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed. Isoform 2 not detected in skeletal muscle and kidney.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed. Isoform 2 not detected in skeletal muscle and kidney.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Sakurai T, et al. Cell 92:573-585(1998)
Limitation
For research use only
Code
OSO00050W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for OREXIN R 2.
Spcs X-react.
Mouse, rat, human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSO00050W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSO00050W
$240USD
|
|
IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of rat spinal cord tissue using Rabbit antibody to Orexin R2: OSO00050W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of rat spinal cord tissue using Rabbit antibody to Orexin R2: OSO00050W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
|
|
IHC on paraffin sections of rat spinal cord tissue using Rabbit antibody to Orexin R2: OSO00050W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
|
|
WB on rat brain lysate using Rabbit antibody to Orexin R2: OSO00050W at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20 |
⚬ Products
Rabbit antibody to Orexin R2
