P2Y1 antibody, P2RY2 antibody, Purinergic receptor antibody, P2Y purinoceptor 1 antibody

Primary Antibodies

Peptides

Histology / Detection

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OVERVIEW
SPECS
CONTROL PEPTIDE
MSDS/DS
IMAGES

Code

OSP00119W

$240USD

ID Tag

Rb1426-250410-WS

Immunogen

A synthetic peptide from the c-terminal region of mouse P2Y1 (P2RY1) conjugated to an immunogenic carrier protein was used as the antigen.

Accession

P2RY1_RAT
P2RY1_MOUSE
P2RY1_GUINEA PIG

Also known

P2Y purinoceptor 1, P2Y1, Purinergic receptor, ATP receptor

Target

FUNCTION: Receptor for extracellular adenine nucleotides such as ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. Repressed by the P2Y1 receptor-specific antagonists A3P5PS, A3P5P and A2P5P. These inhibit calcium ion mobilization and shape change in platelets.
Subcellular location: Membrane; Multi-pass membrane protein.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Tokuyama Y, et al. Biochem. Biophys. Res. Commun. 211:211-218(1995)
2. Leon C, et al. J. Clin. Invest. 104:1731-1737(1999)

Limitation

For research use only

Code

OSP00119W

$240USD

Unit size

100 µl

Conjugate

Unconjugated antibody

Host

NZ white rabbit

Purity

Whole serum

Clonality

Polyclonal

Isotype

Polyclonal, whole serum

Applications

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity

Specific for P2RY1.

Spcs X-react.

Mouse, rat, guinea pig. Other species not yet tested.

Format

Lyophilised

Reconstitution

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code

OSP00119W

$240USD

PDF Data Sheet

- Click to download the Data Sheet

MSDS

Click to download the data sheet - Click to download the Material Safety Data Sheet

Code

OSP00119W

$240USD

IHC on paraffin sections of rat kidney tissue using Rabbit antibody to P2RY1: OSP00119W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of rat lung tissue using Rabbit antibody to P2RY1: OSP00119W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

IHC on paraffin sections of rat skin tissue using Rabbit antibody to P2RY1: OSP00119W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

⚬ Products

Rabbit antibody to P2RY1