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Code
OST00344W
$240USD
ID Tag
Rb1344-140310-WS
Immunogen
A synthetic peptide from aa region 160-210 of mouse TRIM32 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and shares 95% identity with human sequence.
Accession
Also known
TRI32, Tripartite motif-containing protein 32, TRIM32
Target
Function: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha.
Subcellular location: Cytoplasm. Note: Localized in cytoplasmic bodies, usually concentrated around the nucleus.
Tissue specificity: Ubiquitous. High expression in brain.
Subcellular location: Cytoplasm. Note: Localized in cytoplasmic bodies, usually concentrated around the nucleus.
Tissue specificity: Ubiquitous. High expression in brain.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Horn E.J, et al. Carcinogenesis 25:157-167(2004)
2. Albor A, et al. J. Biol. Chem. 281:25850-25866(2006)
2. Albor A, et al. J. Biol. Chem. 281:25850-25866(2006)
Limitation
For research use only
Code
OST00344W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 1000 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for TRIM32.
Spcs X-react.
Mouse, rat. Other species not yet tested but expected to work in human.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OST00344W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00344W
$240USD
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IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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WB on mouse muscle lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:1000 dilution incubated overnight at 4C. Click on eLAB BOOK tab to see the details. |
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IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
⚬ Products
Rabbit antibody to TRIM32 (160-210)
