Rb1290-140210-WS

A synthetic peptide from internal region of human Spastin (Spastic paraplegia 4 protein) conjugated to an immunogenic carrier protein was used as the antigen.

SPAST, Spastic paraplegia 4 protein, ADPSP, FSP2, SPG4

ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. May also play a role in axon growth and the formation of axonal branches.
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein. Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton. Cytoplasm › perinuclear region. Endoplasmic reticulum. Endosome. Nucleus. Cytoplasm › cytoskeleton › spindle. Note: Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons. Isoform 3 is the main endosomal form.
TISSUE SPECIFICITY: Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Hazan J, et al. Nat. Genet. 23:296-303(1999)
2. Claudiani P, et al. Exp. Cell Res. 309:358-369(2005)

For research use only

100 µl

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Specific for Spastin.

Human. Other species not yet tested.

Lyophilised

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

- Click to download the Data Sheet

- Click to download the Material Safety Data Sheet