Anti ATP5A1 antibody, mitochondrial ATP synthase subunit alpha antibody

OVERVIEW
SPECS
CONTROL PEPTIDE
eLAB BOOK
MSDS/DS
IMAGES

Code

OSA00116W

$240USD

ID Tag

Rb1203-051209-WS

Immunogen

A synthetic peptide from aa region 500-550 of human ATP5A1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

ATP5A1_HUMAN
ATP5A1_RAT
ATP5A1_MOUSE

Also known

ATPA, mitochondrial ATP synthase subunit alpha, ATP synthase subunit alpha, mitochondrial, ATP5A, ATP5AL2, ATPM

Target

FUNCTION: Mitochondrial membrane ATP synthase (F₁F₀ ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F₁ - containing the extramembraneous catalytic core, and F₀ - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F₁ is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F₁. Rotation of the central stalk against the surrounding alpha₃beta₃ subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
Subcellular location: Mitochondrion inner membrane. Note: Peripheral membrane protein.
Tissue specificity: Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Kataoka H, et al. Biochim. Biophys. Acta 1089:393-395(1991)

Limitation

For research use only

Code

OSA00116W

$240USD

Unit size

100 µl

Conjugate

Unconjugated antibody

Host

NZ white rabbit

Purity

Whole serum

Clonality

Polyclonal

Isotype

Polyclonal, whole serum

Applications

IHC, WB. A dilution of 1: 2000 for IHC-P and 1:3000 for WB is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity

Specific for ATP5A1.

Spcs X-react.

Human, mouse, rat. Other species not yet tested.

Format

Lyophilised

Reconstitution

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

- Click to see the eLab Book

Code

OSA00116W

$240USD

PDF Data Sheet

- Click to download the Data Sheet

MSDS

Click to download the data sheet - Click to download the Material Safety Data Sheet

Code

OSA00116W

$240USD

IHC-P on paraffin sections of mouse cerebellum.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.

IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

IHC-P on paraffin sections of mouse cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

⚬ Products

Rabbit antibody to ATP5A1 (500-550)