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Code
OST00324W
$260USD
ID Tag
Gt228-030110-WS
Immunogen
A synthetic peptide from aa region 30-100 of human Tyrosine Hydroxylase conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Accession
Also known
TY3H, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, Tyrosine 3 hydroxylase, TH
Target
Function: Plays an important role in the physiology of adrenergic neurons.
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD); also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Subcellular location: Nucleus. Cytoplasm
Tissue specificity: Mainly expressed in the brain and adrenal glands.
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD); also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Subcellular location: Nucleus. Cytoplasm
Tissue specificity: Mainly expressed in the brain and adrenal glands.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Kaneda N, et al. Biochem. Biophys. Res. Commun. 146:971-975(1987)
Limitation
For research use only
Related
products
products
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Code
OST00324W
$260USD
Unit size
150 ul
Conjugate
Unconjugated antibody
Host
Goat
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 200 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for Tyrosine Hydroxylase
Spcs X-react.
Human, rat, mouse, marmoset. Other species not yet tested.
Format
Lyophilised. This product contains 0.02% benzalkonium chloride as a preservative.
Reconstitution
Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OST00324W
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00324W
$260USD
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WB on brain lysates. Blocking with 0.5% LFDM for 1 hour at RT; Primary antibody used at 1:500 dilution incubated overnight at 4C. |
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IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of mouse brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of mouse brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of mouse olfactory. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human large intestine. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of human brain. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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Goat antibody to Tyrosine Hydroxylase (30-100)
