Code
OSC00191W
$240USD
ID Tag
Rb1161-260909-WS
Immunogen
A synthetic peptide from c-terminal region of human Neuronal acetylcholine receptor subunit alpha-10 conjugated to blue carrier protein was used as the antigen. The antigen shares 85% identity with mouse and rat sequences.
Accession
Also known
ACH10, Neuronal acetylcholine receptor subunit alpha-10, Nicotinic acetylcholine receptor subunit alpha-10, NACHR alpha-10, NACHRA10
Target
FUNCTION: Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.
Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction ⺠synapse ⺠postsynaptic cell membrane; Multi-pass membrane protein.
Tissue specificity: Expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Sgard F, et al. Mol. Pharmacol. 61:150-159(2002)
Limitation
For research use only
Code
OSC00191W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for CHRNA10.
Spcs X-react.
Human. Other species not yet tested but expected to work in rat and mouse.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.