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Code
OSF00004W
$240USD
ID Tag
Gt205-101009-WS
Immunogen
A synthetic peptide from the c-terminal region of human Fibrillarin (FIB1) conjugated to an immunogenic carrier protein was used as the antigen.
Accession
Also known
FBRL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, FIB1, FLRN
Target
FUNCTION: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.
Involvement in disease: Antisera from circa 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
SUBCELLULAR LOCATION: Nucleus ⺠nucleolus. Note: Fibrillar region of the nucleolus
Involvement in disease: Antisera from circa 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
SUBCELLULAR LOCATION: Nucleus ⺠nucleolus. Note: Fibrillar region of the nucleolus
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Grimwood J, et al. Nature 428:529-535(2004)
2. Yanagida M, et al. J. Biol. Chem. 279:1607-1614(2004)
3. Choudhary C, et al. Science 325:834-840(2009)
2. Yanagida M, et al. J. Biol. Chem. 279:1607-1614(2004)
3. Choudhary C, et al. Science 325:834-840(2009)
Limitation
For research use only
Code
OSF00004W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
Goat
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for Fibrillarin.
Spcs X-react.
Human, rat, mouse, xenopus. Not yet tested in other species.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSF00004W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
⚬ Products
Goat antibody to Fibrillarin
