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Code
OSV00069W
$240USD
ID Tag
Rb1163-260909-WS
Immunogen
A synthetic peptide from aa region 2-50 of human VGluT1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Accession
Also known
Vesicular glutamate transporter 1, VGlU1, Solute carrier family 17 member 7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Bnpi
Target
FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ni B, et al. Proc. Natl. Acad. Sci. U.S.A. 91:5607-5611(1994).
Limitation
For research use only
Related
products
products
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Code
OSV00069W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
WB, IHC-P, IHC-Fr, ICC, Flow Cyt, ICC/IF. A dilution of 1: 6000 is recommended for WB and 1:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for VGLUT1 (both isoforms 1 and 2).
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OSV00069W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSV00069W
$240USD
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IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin. |
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WB on rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:6000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.1 |
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IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50): OSV00069W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 2000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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Rabbit antibody to VGluT1 (2-50)
