|
|
Code
OSC00164W
$260USD
ID Tag
Rb1024-280609-WS
Immunogen
A synthetic peptide from mouse Catalase conjugated to blue carrier protein was used as the antigen. The antigen is homologous in many other species including rat, human, marmoset, zebrafish, xenopus and guinea pig.
Accession
Also known
CATA, CAT
Target
FUNCTION: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Defects in CAT are the cause of acatalasia (ACATLAS); also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
SUBCELLULAR LOCATION: Peroxisome
Defects in CAT are the cause of acatalasia (ACATLAS); also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
SUBCELLULAR LOCATION: Peroxisome
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Quan F, et al. Nucleic Acids Res. 14:5321-5335(1986)
Limitation
For research use only
Code
OSC00164W
$260USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 2000 is recommended for IHC-P and 1: 7000 for WB. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for Catalase.
Spcs X-react.
Mouse, rat, human, xenopus, zebrafish. Not yet tested in other species.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OSC00164W
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSC00164W
$260USD
|
|
WB on rat liver lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:7000 dilution incubated overnight at 4C. Click on eLAB BOOK tab to see the details. |
|
|
IHC-P on paraffin sections of human liver. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
|
|
IHC-P on paraffin sections of human liver. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
|
|
IHC-P on paraffin sections of human liver. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
|
|
IHC-P on paraffin sections of rat liver. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
⚬ Products
Rabbit antibody to Catalase
