Anti Catalase antibody, Peroxisome Marker antibody, Peroxisome Marker

Primary Antibodies

ABC Transporters

Autophagy

Cancer

Cellular

Immunology

Reporter Genes & Tags

Reproduction

SLC & Regulators

Subcellular Markers

Toxins

Histology / Detection

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OVERVIEW
SPECS
CONTROL PEPTIDE
eLAB BOOK
MSDS/DS
IMAGES

Code

OSC00164W

$260USD

ID Tag

Rb1024-280609-WS

Immunogen

A synthetic peptide from mouse Catalase conjugated to blue carrier protein was used as the antigen. The antigen is homologous in many other species including rat, human, marmoset, zebrafish, xenopus and guinea pig.

Accession

CATL_MOUSE
CATL_HUMAN
CATL_RAT
CATL_DANIO RERIO

Also known

CATA, CAT

Target

FUNCTION: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Defects in CAT are the cause of acatalasia (ACATLAS); also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
SUBCELLULAR LOCATION: Peroxisome

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Quan F, et al. Nucleic Acids Res. 14:5321-5335(1986)

Limitation

For research use only

Code

OSC00164W

$260USD

WB on rat liver lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:7000 dilution incubated overnight at 4C. Click on eLAB BOOK tab to see the details.

IHC-P on paraffin sections of human liver. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

IHC-P on paraffin sections of rat liver. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

⚬ Products

Rabbit antibody to Catalase