Dectin 2 antibody, Dectin2 antibody, Dectin-2 antibody

OVERVIEW
SPECS
CONTROL PEPTIDE
MSDS/DS
IMAGES

Code

OSD00022W

$300USD

ID Tag

Rb1012-280609-WS

Immunogen

A synthetic peptide from mouse Dectin 2 conjugated to an blue carrier protein was used as the antigen.

Accession

DECTIN2_MOUSE

Also known

Dendritic cell-associated lectin 2, DC-associated C-type lectin 2, CLEC6A, Dectin 2, C-type lectin superfamily member 10, CLECSF10, DECTIN2

Target

FUNCTION: May be involved in regulating immune reactivity .
SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
TISSUE SPECIFICITY: Expressed by the XS52 DC (dendritic cell) line. Expressed constitutively by the epidermis, and skin resident DC appear to be the major source of this expression. Expressed in the spleen and thymus. Expression was undetectable in non-DC lines, including macrophage lines (J774 and Raw), T-cell lines (7-17, HDK-1, and D10), B-cell hybridoma (5C5), a keratinocyte line (Pam 212), and a fibroblast line (NS01).

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Ariizumi K, et al. J. Biol. Chem. 275:11957-11963(2000)

Limitation

For research use only

Code

OSD00022W

$300USD

IHC-P on paraffin sections of mouse kidney.
The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions.
Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin.

IHC-P on paraffin sections of mouse kidney. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

⚬ Products

Rabbit antibody to Dectin-2