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Code
OST00297W
$260USD
ID Tag
Rb984-280609-WS
Immunogen
A synthetic peptide from aa region 830-880 of human TLR9 conjugated to blue carrier protein was used as the antigen.
Accession
Also known
Toll-like receptor 9
Target
FUNCTION: Participates in the innate immune response to microbial agents. Detects the unmethylated cytidine-phosphate-guanosine (CpG) motifs present in bacterial DNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
TISSUE SPECIFICITY: Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
TISSUE SPECIFICITY: Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Du X, et al. Eur. Cytokine Netw. 11:362-371(2000)
Limitation
For research use only
Related
products
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Code
OST00297W
$260USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB, Flow Cyt. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for TLR9.
Spcs X-react.
Human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Code
OST00297W
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00297W
$260USD
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IF on human plasmacytoid dendritic cells. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to TLR9 (830-880) ( OST00297W) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. |
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IF on human plasmacytoid dendritic cells. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to TLR9 (830-880) ( OST00297W) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. |
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IF on human plasmacytoid dendritic cells. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to TLR9 (830-880) ( OST00297W) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. |
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WB on Monomac 6 cell lysate (boiled and reduced), using Rabbit antibody to TLR9 (830-880) (OST00297W). SDS-PAGE: 10% Blocking: 1x PBS containing 3% low fat dry milk and 0.1% Tween-20 with shake for 1 hour at RT Washing buffer: 5 times; 1xPBST (0.1% Tween-20) Primary antibody: dilution: 1:500 incubated overnight at 4C Secondary antibody (HRP-Gt anti Rb): dilution: 1:10,000 incubated 1 h at RT Detection: ECL on C-Digit from Licore set at high resolution |
⚬ Products
Rabbit antibody to TLR9 (830-880)
