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Code
OSA00087W
$240USD
ID Tag
Rb968-280609-WS
Immunogen
A synthetic peptide from the c-terminal region of rat Aquaporin 3 (AQP3) conjugated to an immunogenic carrier protein was used as the antigen.
Accession
Also known
Aquaporin-3, AQP-3, AQP3, Aquaporin 3, Aquaporin3
Target
FUNCTION: Forms a water-specific channel that provide kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. May function as a water and urea exit mechanism in antidiuresis in collecting duct cells. Also slightly permeable to urea and glycerol. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
Tissue specificity: Renal medulla and colon. Predominantly in the inner medulla.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
Tissue specificity: Renal medulla and colon. Predominantly in the inner medulla.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Echevarria M, et al. Proc. Natl. Acad. Sci. U.S.A. 91:10997-11001(1994)
2. Ishibashi K, et al. Proc. Natl. Acad. Sci. U.S.A. 91:6269-6273(1994)
2. Ishibashi K, et al. Proc. Natl. Acad. Sci. U.S.A. 91:6269-6273(1994)
Limitation
For research use only
Related
products
products
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Code
OSA00087W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for AQP3.
Spcs X-react.
Rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSA00087W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSA00087W
$240USD
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to AQP3: OSA00087W. |
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to AQP3: OSA00087W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to AQP3: OSA00087W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to AQP3: OSA00087W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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