Rb931-240509-WS

A synthetic peptide from the c-terminal region of rat Orai1 (TMEM142a) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in mouse and human.

ORAI1_RAT
ORAI1_MOUSE
ORAI1_HUMAN

Orai-1, TMEM142a, CRACM1, Calcium release-activated calcium channel protein 1, Transmembrane protein 142A

FUNCTION: Ca²⁺ release-activated Ca²⁺ (CRAC) channel subunit which mediates Ca²⁺ influx following depletion of intracellular Ca²⁺ stores and channel activation by the Ca²⁺ sensor, STIM1. CRAC channels are the main pathway for Ca²⁺ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Defects in ORAI1 are a cause of severe combined immunodeficiency with CRAC channel dysfunction (CRAC-SCID). SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Carninci P, et al. Science 309:1559-1563(2005)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)

For research use only

100 µl

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Specific for Orai1.

Human, rat, mouse. Other species not yet tested.

Lyophilised

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

- Click to download the Data Sheet

- Click to download the Material Safety Data Sheet