⚬ Services
Code
OSK00032W
$260USD    Buy Now 

ID Tag
Rb903-260409-WS

Immunogen
A synthetic peptide from the c-terminal region of human KCNN4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Accession

Also known
Intermediate conductance calcium-activated potassium channel protein 4, SK4, KCa4, IKCa1, IK1, IK1, IKCA1, KCA4, SK4

Target
Function: Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Widely expressed in non-excitable tissues.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Joiner W.J, et al. Proc. Natl. Acad. Sci. U.S.A. 94:11013-11018(1997)

Limitation
For research use only

Related
products
 
Code Product Name
OSP00156G Rabbit antibody to KCNN4
 

Code
OSK00032W
$260USD    Buy Now 

Unit size
100 ul

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for KCNN4.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSK00032W
$260USD    Buy Now 


Rabbit antibody to KCNN4 IHC on paraffin sections of rat skin using Rabbit antibody to KCNN4: OSK00032W.
HIER: 1 mM EDTA, pH 8 for 20 min using Thermo's PT Module
Detection was done using Novolink HRP polymer from Leica following manufacturer's instruction
Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)
Sections were counterstained with Harris Hematoxylin.

⚬ Products
Rabbit antibody to KCNN4
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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