⚬ Services
Code
OSA00076W
$240USD    Buy Now 

ID Tag
Sh134-070309-WS

Immunogen
A synthetic peptide from the c-terminal of human beta, gamma, kappa Actin (ACTB, ACTG, ACTK) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.

Accession

Also known
ACTB, ACTG, ACTK

Target
FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Erba H.P, et al. Nucleic Acids Res. 14:5275-5294(1986).
2. Chang K.-W, et al. Hepatol. Res. 36:33-39(2006).
3. Zahedi R.P, et al. J. Proteome Res. 7:526-534(2008).

Limitation
For research use only

Related
products
 
Code Product Name
OSA00077G Sheep antibody to Actin
 

Code
OSA00076W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
Sheep

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Actin.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Sheep antibody to Actin
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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