⚬ Services
Code
OSR00320G
$240USD    Buy Now 

ID Tag
Rb789-140209-G

Immunogen
A synthetic peptide from aa region 130-180 of human RhoF conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Accession

Also known
Rho family GTPase Rif, Rho in filopodia, ARHF, RIF, Rho in filopodia

Target
Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential. Cytoplasm › cytoskeleton.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Ellis S, et al. Curr. Biol. 10:1387-1390(2000)
2. Ota T, et al. Nat. Genet. 36:40-45(2004)

Limitation
For research use only

Related
products

Code
OSR00320G
$240USD    Buy Now 

Unit size
500 µg

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for RhOF.

Spcs X-react.
Human, rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to RhoF (130-180)
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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