Sh111-060908-G

A synthetic peptide from the phospho S473 of mouse AKT (RAC-alpha serine/threonine-protein kinase, PKB, RAC) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, human and chicken AKT1.

AKT1_MOUSE
AKT1_RAT
AKT1_HUMAN
AKT1_CHICKEN

RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, PKB, RAC, AKT1

FUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.
TISSUE SPECIFICITY: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Cong L.N, et al. Mol. Endocrinol. 11:1881-1890(1997).
2. Pekarsky Y, et al. Proc. Natl. Acad. Sci. U.S.A. 97:3028-3033(2000).
3. Olsen J.V, et al. Cell 127:635-648(2006).

For research use only

	Code Product Name OSA00060W Sheep antibody to phospho S473 of AKT

500 µg

Unconjugated antibody

Sheep

IgG

Polyclonal

Polyclonal, IgG

IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. This antibody is also very likely to recognise the unphosphorylated form.

Specific for AKT1, AKT2, AKT3.

Mouse, rat, human. Other species not yet tested.

Lyophilised

Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

- Click to download the Data Sheet

- Click to download the Material Safety Data Sheet