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Code
OST00267W
$240USD
ID Tag
Rb812-140209-WS
Immunogen
A synthetic peptide from aa region 750-800 of mouse TPC1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and shares 98% identity with human sequence.
Accession
Also known
Two pore calcium channel protein 1, Voltage-dependent calcium channel protein TPC1, TPCN1
Target
Function: May function as one of the major voltage-gated Ca2+ channel (VDCC) across the plasma membrane.
Subcellular location: Membrane; Multi-pass membrane protein.
Tissue specificity: Highest expression found in the heart and kidney, and lowest expression found in the spleen.
Subcellular location: Membrane; Multi-pass membrane protein.
Tissue specificity: Highest expression found in the heart and kidney, and lowest expression found in the spleen.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Perelygin A.A, et al. Proc. Natl. Acad. Sci. U.S.A. 99:9322-9327(2002)
2. Carninci P, et al. Science 309:1559-1563(2005)
2. Carninci P, et al. Science 309:1559-1563(2005)
Limitation
For research use only
Related
products
products
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Code
OST00267W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.
Specificity
Specific for TPC1.
Spcs X-react.
Mouse, rat, human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OST00267W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00267W
$240USD
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WB on rat brain and spinal cord lysate using Rabbit antibody to TPC1 (750-800) (OST00267W).
Dilution: 1: 500, incubated overnight at 4C in MPX from LiCore.
Blocking buffer: 0.2 µm filtered 0.5% LFDM in 1x PBS containing 0.01% Tween-20
Washing buffer: 1x PBS
Detection: ECL using C-Digit from LiCore |
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IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) (OST00267W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) (OST00267W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). |
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IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) (OST00267W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). |
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IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) (OST00267W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). |
⚬ Products
Rabbit antibody to TPC1 (750-800)
